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TOYOPEARL Phenyl-650S

TOYOPEARL Phenyl-650S is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Phenyl ligand group.  TOYOPEARL Phenyl-650S resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TOYOPEARL Phenyl-650S resin has a 100 nm pore size and a 35 µm bead size and is the third most hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Phenyl-650S resin can be used for high resolution intermediate purification and polishing process steps. The primary applications of this resin are for the separation of proteins, their isoforms, and aggregate removal. If greater resolution is needed, the smaller particle TSKgel Phenyl-5PW resins may be used. If lower resolution is desired, the TOYOPEARL Phenyl-650M or TOYOPEARL Phenyl-650C resins may be used. As both the TOYOPEARL Phenyl-650 and TSKgel Phenyl-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL Phenyl-650S resin has a dynamic binding capacity for lysozyme of 30 to 50 g/L. TOYOPEARL Phenyl-650S resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa. 

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
0043152TOYOPEARL Phenyl-650S25 mL35  253
0014477TOYOPEARL Phenyl-650S100 mL35  789
0014784TOYOPEARL Phenyl-650S1 L35  -
0014935TOYOPEARL Phenyl-650S5 L35  -
          

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 35 µm 
Ligand type: Phenyl
DBC: 30 - 50 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years

Structure:

HIC_Phenyl-650C-M-S_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
 
 
            

Pressure-flow Curves for TOYOPEARL Phenyl-650 Resins of Various Particle Sizes


HIC_Phenyl-650_Fig9.png

Resins: A. TOYOPEARL Phenyl-650S
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Phenyl-650C
Column size: 25 mm ID × 25 cm
Mobile phase: 2.0 mol/L (NH4)2SO4
Flow rate: as indicated in figure

 

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
RIS0015 TOYOPEARL Phenyl-650Resin Information Sheet
P/NDescriptionPrice ($) 
0045122MiniChrom TOYOPEARL Phenyl-650S265
0043150TOYOPEARL HICPAK HP666