Process Media


TOYOPEARL Phenyl-650M is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Phenyl ligand group.  TOYOPEARL Phenyl-650M resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL Phenyl-650M resin has a 100 nm pore size and a 65 µm bead size and is the third most hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Phenyl-650M resin can be used for high throughput capture, intermediate purification, and polishing process steps. The primary applications of this resin are for the separation of proteins, their isoforms, and aggregate removal. If greater resolution is needed, the smaller particle TOYOPEARL Phenyl-650S or TSKgel Phenyl-5PW resins may be used. If lower resolution is desired, the TOYOPEARL Phenyl-650C can be used. As both the TOYOPEARL Phenyl-650 and TSKgel Phenyl-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL Phenyl-650M resin has a dynamic binding capacity for lysozyme of 30 to 50 g/L. TOYOPEARL Phenyl-650M resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0019818TOYOPEARL Phenyl-650M25 mL65  180
0014478TOYOPEARL Phenyl-650M100 mL65  550
0014783TOYOPEARL Phenyl-650M1 L65  -
0014943TOYOPEARL Phenyl-650M5 L65  -
0018364TOYOPEARL Phenyl-650M50 L65  -

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 65 µm 
Ligand type: Phenyl
DBC: 30 - 50 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years




Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)


Pressure-flow Curves for TOYOPEARL
Phenyl-650 Resins of Various Particle Sizes


Resins: A. TOYOPEARL Phenyl-650S
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Phenyl-650C
Column size: 25 mm ID × 25 cm
Mobile phase: 2.0 mol/L (NH4)2SO4
Flow rate: as indicated in figure


Selectivity Comparison of Phenyl-type Resins


Resins: A. TOYOPEARL Phenyl-600M
B. TOYOPEARL Phenyl-650M
C. Competitor Phenyl Agarose
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: 1.0 g/L of: 1. ribonuclease A, 2. lysozyme
Load volume: 100 µL


Code #DescriptionLiterature Type
AN002 Scale-up of Anti-TSH IgG Purification from ToyoScreen to Semi-Preparative ColumnsApplication Note
AN117 Simple and Effective Method for Purification of DMT-on Oligonucleotides using Hydrophobic Interaction Chromatography ResinsApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PD05 Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic ProductsPerformance Data
PD16 Stability of TOYOPEARL Ion Exchange and Hydrophobic Interaction Chromatography Resins Stored in 2% Benzyl AlcoholPerformance Data
RIS0015 TOYOPEARL Phenyl-650Resin Information Sheet
TP231 An Effective One-step Purification of 5’-DMT-On Oligonucleotides Using Hydrophobic Interaction ChromatographyTechnical Presentation
P/NDescriptionPrice ($) 
OC41MDHIC-96Resin Seeker TOYOPEARL HIC 96-well plate kit546
0045235SkillPak HIC 1 mL column library325
0045237SkillPak HIC 1 mL column library325
0045233SkillPak HIC 1 mL column library270
0045217SkillPak TOYOPEARL Phenyl-650M 1 mL columns (qty. 5)270
0045253SkillPak TOYOPEARL Phenyl-650M 5 mL column223
0021398ToyoScreen HIC Mix Pack, 1 mL (qty. 5)-
0021399ToyoScreen HIC Mix Pack, 5 mL (qty. 5)-
0021374ToyoScreen Phenyl-650M, 1 mL (qty. 6)-
0021375ToyoScreen Phenyl-650M, 5 mL (qty. 6)-
0045037ToyoScreen RoboColumns Phenyl-650M, 200 µL273
0045038ToyoScreen RoboColumns Phenyl-650M, 600 µL357