Process Media


TOYOPEARL Phenyl-650C is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Phenyl ligand group.  TOYOPEARL Phenyl-650C resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL Phenyl-650C resin has a 100 nm pore size and a 100 µm bead size and is the third most hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Phenyl-650C resin can be used for high throughput capture, intermediate purification, and polishing process steps. The primary applications for this resin are for the separation of proteins, their isoforms, and aggregate removal. If greater resolution is needed, the smaller particle TOYOPEARL Phenyl-650M, TOYOPEARL Phenyl-650S or TSKgel Phenyl-5PW resins may be used. As both the TOYOPEARL Phenyl-650 and TSKgel Phenyl-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL Phenyl-650C resin has a dynamic binding capacity for lysozyme of 30 to 50 g/L. TOYOPEARL Phenyl-650C resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0043126TOYOPEARL Phenyl-650C25 mL100  165
0014479TOYOPEARL Phenyl-650C100 mL100  492
0014785TOYOPEARL Phenyl-650C1 L100  -
0014944TOYOPEARL Phenyl-650C5 L100  -


Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm 
Ligand type: Phenyl
DBC: 30 - 50 g/L 
Shipping buffer: 20% ethanol
pH stability: 1-13
Stability data: 10 years




Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)


Pressure-flow Curves for TOYOPEARL Phenyl-650 Resins of Various Particle Sizes


Resins: A. TOYOPEARL Phenyl-650S
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Phenyl-650C
Column size: 25 mm ID × 25 cm
Mobile phase: 2.0 mol/L (NH4)2SO4
Flow rate: as indicated in figure


Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
RIS0015 TOYOPEARL Phenyl-650Resin Information Sheet
P/NDescriptionPrice ($)