TOYOPEARL Butyl-650S

TOYOPEARL Butyl-650S is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Butyl ligand group. TOYOPEARL Butyl-650S resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TOYOPEARL Butyl-650S resin has a 100 nm pore size and a 35 µm bead size and is the second most hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Butyl-650S resin can be used for high resolution intermediate purification and polishing process steps. The primary applications of this resin are for the separation of proteins, their isoforms, and aggregate removal. If lower resolution is desired, the TOYOPEARL Butyl-650M or TOYOPEARL Butyl-650C resins may be used.

TOYOPEARL Butyl-650S resin has a dynamic binding capacity for lysozyme of 30 to 50 g/L. TOYOPEARL Butyl-650S resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0043153	TOYOPEARL Butyl-650S	25 mL	35	254	Inquire
0007476	TOYOPEARL Butyl-650S	100 mL	35	792	Inquire
0014701	TOYOPEARL Butyl-650S	1 L	35	-	Inquire
0007975	TOYOPEARL Butyl-650S	5 L	35	-	Inquire
0018826	TOYOPEARL Butyl-650S	50 L	35	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	35 µm
Ligand type:	Butyl
DBC:	30 - 50 g/L
Shipping buffer:	20% ethanol
pH stability:	1-13
Stability data:	10 years

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Purification of Glycoproteins

Resins:	TOYOPEARL Butyl-650S
Column size:	22 mm ID × 26 cm
Mobile phase:	Buffer A: 40% saturated (NH₄)₂SO₄+ 50 mol/L phosphate, pH 7.0 Buffer B: 50 mol/L phosphate, pH 7.0
Gradient:	A: Load and wash in 100% buffer A B: 50% buffer A with 50% buffer B C: 100% buffer B
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	crude protein from sea hare Aplysia kurodai

Purification and Resolution of Pullanase

Resins:	TOYOPEARL Butyl-600S
Column size:	18 mm ID × 16 cm
Mobile phase:	isocratic elution, 120 mL (NH₄)₂SO₄, 25% saturation in 0.02 mol/L phosphate, pH 7.0, followed by a linear gradient, 224 mL (NH₄)₂SO₄, 25% to 0% saturation, in buffer
Flow rate:	12 cm/hr
Detection:	UV @ 280 nm
Sample:	protein from Bacillus acidopullulyticus
Sample load:	20 mg

For additional applications, please see our applications database.

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
IM14	Instruction Manual -- Principles of Hydrophobic Interaction Chromatography	Instruction Manual
PCAT03	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic Interaction	Product Catalog
RIS0012	TOYOPEARL Butyl-650	Resin Information Sheet

P/N	Description	Price ($)
0045126	MiniChrom TOYOPEARL Butyl-650S	-	Inquire
0043150	TOYOPEARL HICPAK HP	680	Inquire