Process Media


TOYOPEARL Butyl-650S is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Butyl ligand group.  TOYOPEARL Butyl-650S resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TOYOPEARL Butyl-650S resin has a 100 nm pore size and a 35 µm bead size and is the second most hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Butyl-650S resin can be used for high resolution intermediate purification and polishing process steps. The primary applications of this resin are for the separation of proteins, their isoforms, and aggregate removal. If lower resolution is desired, the TOYOPEARL Butyl-650M or TOYOPEARL Butyl-650C resins may be used.

TOYOPEARL Butyl-650S resin has a dynamic binding capacity for lysozyme of 30 to 50 g/L. TOYOPEARL Butyl-650S resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa. 


P/NDescriptionResin VolumeParticle Size (µm)   Price
0043153TOYOPEARL Butyl-650S25 mL35  254
0007476TOYOPEARL Butyl-650S100 mL35  792
0014701TOYOPEARL Butyl-650S1 L35  -
0007975TOYOPEARL Butyl-650S5 L35  -
0018826TOYOPEARL Butyl-650S50 L35  -


Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 35 µm 
Ligand type: Butyl
DBC: 30 - 50 g/L 
Shipping buffer: 20% ethanol
pH stability: 1-13
Stability data: 10 years




Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)


Purification of Glycoproteins


Resins: TOYOPEARL Butyl-650S
Column size: 22 mm ID × 26 cm
Mobile phase: Buffer A: 40% saturated (NH4)2SO4+ 50 mol/L phosphate, pH 7.0
Buffer B: 50 mol/L phosphate, pH 7.0
Gradient: A: Load and wash in 100% buffer A
B: 50% buffer A with 50% buffer B
C: 100% buffer B
Detection: UV @ 280 nm
Temperature: ambient
Sample: crude protein from sea hare Aplysia kurodai

Purification and Resolution of Pullanase


Resins: TOYOPEARL Butyl-600S
Column size: 18 mm ID × 16 cm
Mobile phase: isocratic elution, 120 mL  (NH4)2SO4, 25% saturation in 0.02 mol/L phosphate, pH 7.0, followed by a linear gradient, 224 mL (NH4)2SO4, 25% to 0% saturation, in buffer
Flow rate: 12 cm/hr
Detection: UV @ 280 nm
Sample: protein from Bacillus acidopullulyticus
Sample load: 20 mg

For additional applications, please see our applications database.

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
RIS0012 TOYOPEARL Butyl-650Resin Information Sheet
P/NDescriptionPrice ($) 
0045126MiniChrom TOYOPEARL Butyl-650S-