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TOYOPEARL SuperButyl-550C

TOYOPEARL SuperButyl-550C is based on a 50 nm (500 Å), 100 µm pore size polymethacrylate base material bonded with C4 groups. It has the second highest hydrophobicity of the TOYOPEARL HIC resins. Its pore size is the smallest of our three butyl resins.

Primary applications are for the separation of smaller proteins. Depending on the feedstock's molecular size profile, it is also useful in negative or flow through chromatography. It can be used for capture and intermediate purification process steps.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
0019955TOYOPEARL SuperButyl-550C25 mL100  194
0019956TOYOPEARL SuperButyl-550C100 mL100  599
0019957TOYOPEARL SuperButyl-550C1 L100  -
0019958TOYOPEARL SuperButyl-550C5 L100  -
0019959TOYOPEARL SuperButyl-550C50 L100  -
          

Product Attributes

Base bead: HW-55
Pore size (mean): 50 nm
Particle size (mean): 100 µm 
Ligand type: Butyl
DBC: 52 - 70 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years

Structure:

HIC_SuperButyl-550C_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
 
 
           

Dynamic Binding Capacity Comparision for HIC Resins

Lysozyme

HIC_Ether-650M_PPG-600M_SuperButyl-550C_Hexyl-650_Fig6.png

Resins: binding capacity
(10% breakthrough)
TOYOPEARL Ether 650M 12.5 g/L
TOYOPEARL Phenyl 650M
27.5 g/L
TOYOPEARL Butyl 650M
32.2 g/L
TOYOPEARL Hexyl 650C
33.2 g/L
TOYOPEARL PPG 600M
44.2 g/L
TOYOPEARL SuperButyl 550C
54.3 g/L

 

Resins: A. TOYOPEARL Ether-650M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-650M
D. TOYOPEARL Hexyl-650C
E. TOYOPEARL PPG-600M
F. TOYOPEARL SuperButyl-550C
Column size: 7.8 cm ID × 20 cm
Mobile phase: 1.8 mol/L sodium sulfate +
0.1 mol/L phosphate,pH 7.0
Flow rate: 100 cm/hr (0.8 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: 1 g/L lysozyme
Sample load: as indicated in figure

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PO03 TOYOPEARL Super Butyl-550C, for Hydrophobic Interaction Chromatography of Proteins and PeptidesProduct Overview
RIS0017 TOYOPEARL SuperButyl-550CResin Information Sheet
P/NDescriptionPrice ($) 
OC41MDHIC-96Resin Seeker TOYOPEARL HIC 96-well plate kit546
0021382ToyoScreen SuperButyl-550C, 1 mL (qty. 6)-
0021383ToyoScreen SuperButyl-550C, 5 mL (qty. 6)-