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TOYOPEARL Hexyl-650C

TOYOPEARL Hexyl-650C is based on a 100 nm (1000 Å) pore size, 100 µm polymethacrylate base material bonded with C6 groups. This resin has the highest hydrophobicity of the TOYOPEARL HIC resins. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)		 
0044465TOYOPEARL Hexyl-650C25 mL100  202
0019026TOYOPEARL Hexyl-650C100 mL100  629
0019027TOYOPEARL Hexyl-650C1 L100  -
0019028TOYOPEARL Hexyl-650C5 L100  -
0021973TOYOPEARL Hexyl-650C50 L100  -

       

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm 
Ligand type: Hexyl
DBC: 30 - 50 g/L 
Shipping buffer: 20% ethanol
pH stability: 1-13
Stability data: 10 years

Structure:

HIC_Hexyl-650C_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
 
    

Plasmid DNA Purification


HIC-Hexyl-650C-Fig13_rev.png
Resin: TOYOPEARL Hexyl-650C
Column size: 1.0 cm ID × 30 cm (23.6 mL)
Mobile phase: Buffer A: 3.0 mol/L ammonium sulfate, 10 mmol/L Tris, 1 mmol/L EDTA, pH 7.4
Buffer B: 10 mmol/L Tris, 1 mmol/L EDTA, pH 7.4
Gradient: 3.0 mol/L ammonium sulfate to 1.0 mol/L ammonium sulfate (6 CV)
Flow rate: 153 cm/hr (2.0 mL/min)
Detection: UV @ 254 nm
Samples: Plasmid DNA in 3.0 mol/L ammonium sulfate

For additional applications, please see our applications database.

           

Dynamic Binding Capacity Comparision for HIC Resins

Lysozyme

HIC_Ether-650M_PPG-600M_SuperButyl-550C_Hexyl-650_Fig6.png

Resins: binding capacity
(10% breakthrough)
TOYOPEARL Ether 650M 12.5 g/L
TOYOPEARL Phenyl 650M
 27.5 g/L
TOYOPEARL Butyl 650M
 32.2 g/L
TOYOPEARL Hexyl 650C
 33.2 g/L
TOYOPEARL PPG 600M
 44.2 g/L
TOYOPEARL SuperButyl 550C
 54.3 g/L

 

Resins: A. TOYOPEARL Ether-650M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-650M
D. TOYOPEARL Hexyl-650C
E. TOYOPEARL PPG-600M
F. TOYOPEARL SuperButyl-550C
Column size: 7.8 cm ID × 20 cm
Mobile phase: 1.8 mol/L sodium sulfate +
0.1 mol/L phosphate,pH 7.0
Flow rate: 100 cm/hr (0.8 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: 1 g/L lysozyme
Sample load: as indicated in figure

Code #DescriptionLiterature Type
AN117 Simple and Effective Method for Purification of DMT-on Oligonucleotides using Hydrophobic Interaction Chromatography ResinsApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PD05 Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic ProductsPerformance Data
RIS0014 TOYOPEARL Hexyl-650CResin Information Sheet
TP231 An Effective One-step Purification of 5’-DMT-On Oligonucleotides Using Hydrophobic Interaction ChromatographyTechnical Presentation
P/NDescriptionPrice ($) 
OC41MDHIC-96Resin Seeker TOYOPEARL HIC 96-well plate kit-
0045237SkillPak HIC 1 mL column library-
0045238SkillPak HIC 1 mL column library-
0045234SkillPak HIC 1 mL column library-
0045233SkillPak HIC 1 mL column library284
0045219SkillPak TOYOPEARL Hexyl-650C 1 mL columns (qty. 5)284
0045255SkillPak TOYOPEARL Hexyl-650C 5 mL column235
0043125TOYOPEARL HICPAK-C577
0021378ToyoScreen Hexyl-650C, 1 mL (qty. 6)-
0021379ToyoScreen Hexyl-650C, 5 mL (qty. 6)-
0021398ToyoScreen HIC Mix Pack, 1 mL (qty. 5)-
0021399ToyoScreen HIC Mix Pack, 5 mL (qty. 5)-