Process Media

TSKgel Phenyl-5PW (30)

TSKgel Phenyl-5PW (30) resin is a hydrophobic interaction chromatography resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Phenyl ligand group. TSKgel Phenyl-5PW (30) is more hydrophobic than the Ether-5PW resins and is the best choice for the separation of moderately hydrophobic proteins or monoclonal antibodies. This resin has a 100 nm pore size and a 30 µm bead size.

TSKgel Phenyl-5PW (30) resin has a high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.  TSKgel PW resins vary from Tosoh’s TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW hydrophobic interaction chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than their TOYOPEARL counterparts.

TSKgel Phenyl-5PW (30) resin is best used for high resolution process polishing steps. If a product with less resolution is needed, the larger particle TOYOPEARL Phenyl-650 resins may be used. If higher resolution is required, the TSKgel Phenyl-5PW (20) should be used.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0043177TSKgel Phenyl-5PW (30)25 mL30  381
0014720TSKgel Phenyl-5PW (30)250 mL30  2,756
0014721TSKgel Phenyl-5PW (30)1 L30  -
0017210TSKgel Phenyl-5PW (30)5 L30  -


Product Attributes

Base bead: 5000PW
Pore size (mean): 100 nm
Particle size (mean): 30 µm 
Ligand type: Phenyl
DBC: 10 - 30 g/L 
Shipping buffer: 20% ethanol
pH stability: 1-13
Stability data: 10 years




Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)


Ultra Purification of Target Compound

Seamless scale-up


Resins: A and B. TSKgel Phenyl-5PW
C. TOYOPEARL Phenyl-650M
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: A and B: 60 min linear gradient from 1.8 mol/L to 0 mol/L (NH4)2SO4 in 0.1 mol/L phosphate buffer
C: 240 min linear gradient from 1.8 mol/L to 0 mol/L (NH4)2SO4 in 0.1 mol/L phosphate buffer
Flow rate: A and B. 68 cm/hr (0.50 mL/min)
C. 272 cm/hr (2.0 mL/min)
Detection: UV @ 280 nm
Samples: 1. myoglobin
2. ribonuclease A
3. lysozyme
4. α-chymotrypsinogen
Load volume: 100 µL containing 1 g/L of each protein

For additional applications, please see our applications database.

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PD05 Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic ProductsPerformance Data
RIS0019 TSKgel Phenyl-5PWResin Information Sheet
P/NDescriptionPrice ($) 
0043175TSKgel HICPAK PW, 30 µm 542