TOYOPEARL AF-rProtein L-650F

TOYOPEARL AF-rProtein L-650F is a protein L resin that exhibits the highest binding capacity for antibody fragments that lack a Fc-portion (e.g. Fab, scFv, Dab, etc.).

TOYOPEARL AF-rProtein L-650F resin does not only offer the highest binding capacity, but also has the ability to bind to antibody classes that do not have the affinity to Protein A, such as IgM and IgA.

The selected recombinant protein L ligand used in the TOYOPEARL AF-rProtein L-650F resin is alkali-stable and is therefore able to withstand the high alkaline conditions necessary for clean-in-place (CIP). This resin exhibits stability in various solutions and over a wide pH range, as well as demonstrates excellent pressure-flow rate properties and will withstand back pressures up to 0.2 MPa.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0023486	TOYOPEARL AF-rProtein L-650F	10 mL	45	678	Inquire
0023487	TOYOPEARL AF-rProtein L-650F	25 mL	45	1,171	Inquire
0023488	TOYOPEARL AF-rProtein L-650F	100 mL	45	2,339	Inquire
0023489	TOYOPEARL AF-rProtein L-650F	1 L	45	-	Inquire
0023490	TOYOPEARL AF-rProtein L-650F	5 L	45	-	Inquire

Product Attributes

Pore size (mean):	100 nm
Particle size (mean):	45 µm
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data (estimated):	10 years

Structure:

Separation of Fab and Fc (papain digestion)

Resin:	TOYOPEARL AF-rProtein L-650F
Column size:	5 mm ID × 2.5 cm
Mobile phase:	Buffer A: 0.1 mol/L Na-Citrate, pH 6.5 Buffer B: 0.1 mol/L Citrate, pH 2.05
Gradient:	B = 0% (0 to 1 min) B = 0% to 100% (1 min to 16 min)
Flow rate:	0.5 mL/min (residence time = 1 min)
Detection:	UV @ 280 nm
Injection:	3 mg BI_mAb2 papain digestion

Purification of scFv (mammalian cell culture)

Resin:	TOYOPEARL AF-rProtein L-650F
Column size:	5 mm ID × 3.5 cm (0.5 mL)
Equib. & flash wash:	0.1 mol/L Na-Citrate, pH 6.5
Intermediate wash:	0.1 mol/L Na-Citrate, pH 6.5 + 1 mol/L Arg-HCl, 10% iPrOH
Elute:	0.1 mol/L Citrate, pH 2.3
CIP:	0.05 mol/L NaOH
Flow rate:	0.35 mL/min (residence time = 1.4 min)
Detection:	UV @ 280 nm
Sample:	hu-scFv1 CHO culture sup. 40 mL

For additional applications, please see our applications database.

Highest Dynamic Binding Capacity for Fab

Excellent Stability in 0.1 mol/L NaOH

Widest Affinity for Fab Classes

Species	Class	Affinity
General	Kappa light chain	++
	Lambda light chain	-
	Heavy chain	-
	Fab	++
	ScFv	++
	Dab	++
Human	IgG(1-4)	+
	IgA	+
	IgD	+
	IgE	+
	IgM	+
Mouse	IgG1	+
	IgG2a	+
	IgG2b	+
	IgA	+
	IgM	+
Rat	IgG1	+
	IgG2a,b,c	+
	IgA	+
Chicken	IgM	+
	IgY	+

Code #	Description	Literature Type
AN100	Purification of Fab and scFv using High Binding Capacity TOYOPEARL AF-rProtein L-650F	Application Note
AN103	Capture of a scFv from E. coli using TOYOPEARL AF-rProtein L-650F	Application Note
AN134	Protein L Chromatography Process Transfer for the Continuous Purification of Fab-Fragments	Application Note
BR10	Best-In-Class Bioprocessing Resins	Brochure
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT04	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Affinity	Product Catalog
PO46	TOYOPEARL AF-rProtein L-650F	Product Overview
RIS0010	TOYOPEARL AF-Protein-L-650F	Resin Information Sheet

P/N	Description	Price ($)
OC41MDAFPL-650F	Resin Seeker TOYOPEARL AF-rProtein L-650F 96-well plate kit	-	Inquire
0045228	SkillPak Antibody 1 mL column library	434	Inquire
0045232	SkillPak Best in Class 1 mL column library	430	Inquire
0045266	SkillPak Best in Class 5 mL column library	1,406	Inquire
0045200	SkillPak TOYOPEARL AF-rProtein L-650F 1 mL column	133	Inquire
0045221	SkillPak TOYOPEARL AF-rProtein L-650F 1 mL columns (qty. 5)	662	Inquire
0045257	SkillPak TOYOPEARL AF-rProtein L-650F 5 mL column	384	Inquire
0023494	ToyoScreen AF-rProtein L-650F, 1 mL (qty. 5)	690	Inquire
0023495	ToyoScreen AF-rProtein L-650F, 5 mL (qty. 1)	637	Inquire
0023496	ToyoScreen AF-rProtein L-650F, 5 mL (qty. 5)	2,070	Inquire
0045065	ToyoScreen RoboColumns AF-rProtein L-650F, 200 µL	-	Inquire
0045066	ToyoScreen RoboColumns AF-rProtein L-650F, 600 µL	1,354	Inquire