Process Media

TOYOPEARL AF-rProtein L-650F

TOYOPEARL AF-rProtein L-650F is a protein L resin that exhibits the highest binding capacity for antibody fragments that lack a Fc-portion (e.g. Fab, scFv, Dab, etc.).

TOYOPEARL AF-rProtein L-650F resin does not only offer the highest binding capacity, but also has the ability to bind to antibody classes that do not have the affinity to Protein A, such as IgM and IgA. 

The selected recombinant protein L ligand used in the TOYOPEARL AF-rProtein L-650F resin is alkali-stable and is therefore able to withstand the high alkaline conditions necessary for clean-in-place (CIP). This resin exhibits stability in various solutions and over a wide pH range, as well as demonstrates excellent pressure-flow rate properties and will withstand back pressures up to 0.2 MPa.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0023486TOYOPEARL AF-rProtein L-650F10 mL45  678
0023487TOYOPEARL AF-rProtein L-650F25 mL45  1,171
0023488TOYOPEARL AF-rProtein L-650F100 mL45  2,339
0023489TOYOPEARL AF-rProtein L-650F1 L45  -
0023490TOYOPEARL AF-rProtein L-650F5 L45  -


Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 45 µm
Shipping buffer: 20% ethanol
pH stability: 2-13
Stability data (estimated): 10 years




Separation of Fab and Fc (papain digestion)


Resin: TOYOPEARL AF-rProtein L-650F
Column size: 5 mm ID × 2.5 cm
Mobile phase: Buffer A: 0.1 mol/L Na-Citrate, pH 6.5
Buffer B: 0.1 mol/L Citrate, pH 2.05
Gradient: B = 0% (0 to 1 min)
B = 0% to 100% (1 min to 16 min)
Flow rate: 0.5 mL/min (residence time = 1 min)
Detection: UV @ 280 nm
Injection: 3 mg BI_mAb2 papain digestion

Purification of scFv (mammalian cell culture)


Resin: TOYOPEARL AF-rProtein L-650F
Column size: 5 mm ID × 3.5 cm (0.5 mL)
Equib. & flash wash: 0.1 mol/L Na-Citrate, pH 6.5
Intermediate wash: 0.1 mol/L Na-Citrate, pH 6.5 +
1 mol/L Arg-HCl, 10% iPrOH
Elute: 0.1 mol/L Citrate, pH 2.3
CIP: 0.05 mol/L NaOH
Flow rate: 0.35 mL/min (residence time = 1.4 min)
Detection: UV @ 280 nm
Sample: hu-scFv1 CHO culture sup. 40 mL

For additional applications, please see our applications database.


Highest Dynamic Binding Capacity for Fab


Excellent Stability in 0.1 mol/L NaOH


Widest Affinity for Fab Classes

Species Class Affinity
General Kappa light chain ++

Lambda light chain -

Heavy chain -

Fab ++

ScFv ++

Dab ++
Human IgG(1-4) +

IgA +

IgD +

IgE +

IgM +
Mouse IgG1 +
IgG2a +
IgG2b +
IgA +

IgM +
Rat IgG1 +

IgG2a,b,c +
IgA +
Chicken IgM +
IgY +
Code #DescriptionLiterature Type
AN100 Purification of Fab and scFv using High Binding Capacity TOYOPEARL AF-rProtein L-650FApplication Note
AN103 Capture of a scFv from E. coli using TOYOPEARL AF-rProtein L-650FApplication Note
AN134 Protein L Chromatography Process Transfer for the Continuous Purification of Fab-FragmentsApplication Note
BR10 Best-In-Class Bioprocessing ResinsBrochure
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
PO46 TOYOPEARL AF-rProtein L-650FProduct Overview
RIS0010 TOYOPEARL AF-Protein-L-650FResin Information Sheet
P/NDescriptionPrice ($) 
OC41MDAFPL-650FResin Seeker TOYOPEARL AF-rProtein L-650F 96-well plate kit-
0045228SkillPak Antibody 1 mL column library434
0045232SkillPak Best in Class 1 mL column library 430
0045266SkillPak Best in Class 5 mL column library1,406
0045200SkillPak TOYOPEARL AF-rProtein L-650F 1 mL column133
0045221SkillPak TOYOPEARL AF-rProtein L-650F 1 mL columns (qty. 5)662
0045257SkillPak TOYOPEARL AF-rProtein L-650F 5 mL column384
0023494ToyoScreen AF-rProtein L-650F, 1 mL (qty. 5)690
0023495ToyoScreen AF-rProtein L-650F, 5 mL (qty. 1)637
0023496ToyoScreen AF-rProtein L-650F, 5 mL (qty. 5)2,070
0045065ToyoScreen RoboColumns AF-rProtein L-650F, 200 µL-
0045066ToyoScreen RoboColumns AF-rProtein L-650F, 600 µL1,354