Multi Column Chromatography

Octave PRO MCC Skid

The OctaveTM PRO is a GMP-ready multi-column chromatography (MCC) system for the purification of biopharmaceuticals at the clinical and commercial scale. The Octave PRO is designed for direct scale-up after process development on the Octave BIO, and both systems offer the same method-execution capabilities. The Octave PRO enables ultimate process versatility and agility to address the most challenging downstream bottlenecks.

  • GMP-ready skid using reliable, patented Octave technology
  • Specifically designed for typical clinical and commercial scale purification processes of 2000 L bioreactors within 1-2 shift times
  • Flow rates ranging from 0.1 L/min to 2.5 L/min
  • Up to eight columns
  • Six pumps, six inlets, six outlets
  • Single use flow path
  • Integrated UV, conductivity, and pH sensors (4 of each)
  • Complete data recording and analysis
  • Includes PROComposer™ Method Creator and Method Wizard applications
  • 21 CFR Part 11 compliant PROController™ control system
  • Dedicated SkillPak PRO pre-packed columns for MCC applications are available

Octave PRO


0041200Octave PRO MCC Skid-


System Architecture

Description Specifications
Dimensions (W / D / H) 213 / 115 / 219 cm, (198 cm without alarm tower)
Weight 450 kg
Operating flow rate 1 – 150 L/hr (2.5 L/min)
Maximum operating pressure 6 bar (87 psi)
Valve Pressure 8 bar (116 psi)
Gas inlet pressure 9 bar (130 psi)
Column Positions/Connections 1 to 8 columns, 3/4 in. sanitary clamps
Valve block 1 single-use assembly containing 104 pneumatic two-way valves
Inlet number 6
Outlet number 6
Pumps 6; 4-piston-diaphragm pump, flow rate ranges 1-150 L/h
Flowmeters 6; 1 after each pump, flow rate ranges 0.01-8 L/min
Pressure sensors 6; 1 after each pump, measuring range 1-10 bar
Outlet sensors 4 assignable single-use combination UV/conductivity/pH
UV sensors UV 280 nm & 305 nm
Conductivity sensors CND range 0–850 mS/cm
pH sensors pH range 0-14

Single-Use Flowkit

Item Description
Inlet Connections 6 Aseptiquik® G
Outlet Connections 6 Aseptiquik® G
Pumps 6 Quattroflow® EZ-Set Pump Chamber quaternary diaphragm single-use heads
Pressure sensors 6 single-use gauge tees (TS1, 1 after each pump)
Flow sensors 6 single-use; ultrasonic (1 after each pump)

Single-Use Materials of Construction

Component Wetted Materials
Pump head Polypropylene (PP), thermoplastic elastomer (TPE), ethylene propylene diene monomer (EPDM)
Pressure adapter PP, TPE
Flow sensor PP
Valve block assembly USP class VI compliant polyetherimide (PEI), polyvinylidene fluoride (PVDF), polyfluoroalkoxy (PFA)
Optical/conductivity/pH flow cell Quartz (UV-transparent), EPDM, stainless steel 1.4435 (SS 316L), polyphenylsulfone (PPSU)
Fittings PEEK, platinum cured silicone, PP
1/4 in. ID polybraided platinum cured silicone, pump inlet is weldable 1/4 in. ID Advantaflex biopharmaceutical grade thermoplastic elastomer (TPE)
Gamma irradiation dose 25-40 kGy

Electrical Requirements

Parameter North America EMEA
Voltage 120V;1 phase 220-240V; 1 phase
Amps 30A 15A
Frequency 60 Hz 50 Hz
Number of Cords 1 1
Type of Plug NEMA L5-30P, with ground Must meet local code, with ground
Code #DescriptionLiterature Type
AN142 Multi-Column Chromatography Implementation and Scale-Up to Clinical Scale with the Octave™ MCC SystemsApplication Note
AN142 Multi-Column Chromatography Implementation and Scale-Up to Clinical Scale with the Octave™ MCC SystemsApplication Note
BR13 Octave™ Multi-Column Chromatography- A holistic solution for process intensificationBrochure
DA0101A-G Octave™ PRO Specification SheetInstrument Specifications
P/NDescriptionPrice ($) 
0041202Octave PRO SU Flowkit-