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TSKgel® PWxl Columns

TSKgel® PWXL Columns for Analysis of Water-Soluble Polymers

TSKgel® PWXL columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for these columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel® PW columns page for typical samples and suitable eluents.

The figures below show the calibration curves for all TSKgel® PWXL columns. 

PWXL_Cal-curves1a.png
 PWXL_Cal-curves2.png
Columns:




Mobile phase:
Flow rate:
Detection:		 TSKgel® PWXL columns,
all 7.8 mm ID × 30 cm
A. G2500PWXL, B. G3000PWXL
C. G4000PWXL, D. G5000PWXL,
E. G6000PWXL, F. GMPWXL
distilled H20
1.0 mL/min
RI
Columns:


Mobile phase:
Flow rate:
Detection:
Samples:		 TSKgel® PWXL columns, all 7.8 mm ID × 30 cm
1. G3000PWXL,  2. G4000PWXL,
3. G5000PWXL, 4. G6000PWXL, 5. GMPWXL
0.2 mol/L phosphate buffer, pH 6.8
1.0 mL/min
UV @280 nm
25 °C
a. thyroglobulin (6.6 × 105 Da)
b. g-globulin (1.5 × 105 Da)
c. albumin (6.7 × 104 Da)
d. ovalbumin (4.3 × 104 Da)
e. b-lactoglobulin (3.6 × 104 Da)
f. myoglobin (1.69 × 104 Da)
g. cytochrome C (1.24 × 104 Da)
G-DNA-PW_Cal-curves_a.png G-Oligo_PW_Cal-curves_b.png  
 SuperOligoPW_Cal-curves_b.png
Column:


Mobile phase:




Flow rate:
Detection:
Sample:		 TSKgel® G-DNA-PW,
10 µm,
7.8 mm ID × 30 cm × 4  
0.2 mol/L  H2O  with
0.3 mol/L NaCl in
0.1 mol/LTris-HCl,
pH 7.5, + 1 mmol/L EDTA
0.15 mL/min
UV @ 260 nm
Eco RI and Bst NI-cleaved pBR322 DNA, void volume determined with λ-DNA
Column:



Mobile phase:
Flow rate:
Detection:
Sample:		 TSKgel® G-Oligo-PW,
7 µm,
7.8 mm ID × 30 cm × 2
distilled H2O
1.0 mL/min
UV @ 260 nm
hydrolyzed
β-cyclodextrin
Column:

Mobile phase:
Flow rate:
Detection:
Temperature:
Samples:		 TSKgel® SuperOligoPW, 
6.0 mm ID × 15 cm
H2O
0.60 mL/min
RI
25 °C
PEO, PEG and ethylene glycol

Specialty TSKgel® PWXL Columns

TSKgel® G-DNA-PW is available for the analysis of large polynucleotides, such as DNA and RNA fragments of 500-5,000 base pairs.

TSKgel® G-Oligo-PW is the preferred column for the high resolution analysis of aqueous nonionic and cationic oligmers and oligosaccharides such as hydrolyzed cyclodextrins. 

TSKgel® SuperOligoPW contains smaller particles and semi-micro dimensions compared to the TSKgel G-Oligo-PW column, enabling high speed separations with high resolution of aqueous oligomers.

The mixed-mode column, TSKgel® GMPWXL, has an extended linear calibration range, suitable for samples with a broad molar mass (MM) distribution and unknown samples.

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 7 µm  
Pore size (mean): < 20 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 < 3,000 Da             Visit the TSKgel® G2500PWXL product page.
Max. % Organic: 20%           
Shipping Solvent: water

   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 7 µm  
Pore size (mean): 20 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 < 40,000 Da             Visit the TSKgel® G3000PWXL product page.
Max. % Organic: 20%           
Shipping Solvent: water

   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 10 µm  
Pore size (mean): < 50 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 2,000 - 300,000 Da             Visit the TSKgel® G4000PWXL product page.
Max. % Organic: 20%           
Shipping Solvent: water

   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 10 µm  
Pore size (mean): 100 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 4,000 - 800,000 Da             Visit the TSKgel® G5000PWXL product page.
Max. % Organic: 20%           
Shipping Solvent: water

   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 13 µm  
Pore size (mean): > 100 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 40,000 - 8,000,000 Da             Visit the TSKgel® G6000PWXL product page.
Max. % Organic: 20%           
Shipping Solvent: water

   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 10 µm  
Pore size (mean): > 100 nm  
Working Range:  500 - 5,000 bp DNA  
Calibration Range 
(polyethylene glycols 
and oxides):		 40,000 - 8,000,000 Da             Visit the TSKgel® G-DNA-PW product page.
Max. % Organic: 20%           
Shipping Solvent: water

   

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 13 µm  
Pore size (mean): 10 - 100 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 1,000 - 8,000,000 Da             Visit the TSKgel® GMPWXL product page.
Max. % Organic: 20%           
Shipping Solvent: water

 

Base Material: hydroxylated polymethacylate  
Particle Size (mean): 7 µm  
Pore size (mean): 12.5 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 up to 3,000 Da             Visit the TSKgel® G-Oligo-PW product page.
Max. % Organic: 20%           
Shipping Solvent: water

   

Base Material: polymethacylate  
Particle Size (mean): 3 µm  
Pore size (mean): 12.5 nm  
Calibration Range 
(polyethylene glycols 
and oxides):		 100 - 3,000 Da             Visit the TSKgel® SuperOligoPW product page.
Max. % Organic: 20%           
Shipping Solvent: water