TSKgel® HIC-ADC Columns – for antibody-drug-conjugate (ADC) analysis
The name gives it away: TSKgel® HIC-ADC columns are hydrophobic interaction chromatography (HIC) columns designed for the analysis of Antibody-Drug Conjugates (ADCs). Tosoh has developed a new particle construction to achieve exceptional separation of variably conjugated ADC variants. This next-generation breakthrough in non-porous particle technology makes DAR analysis more reliable and improves separation efficiency. The TSKgel® HIC-ADC columns set a new industry standard with respect to the following characteristics.
- Superior separation performance
- Fast analyses (e.g. DAR determination in 7 minutes)
- Reliability and lifetime
- Ligand selection for diverse molecules: Phenyl (high hydrophobicity), Butyl (mid hydrophobicity)
The columns simplify method development and tech-transfer processes for DAR analyses, enhancing both the accuracy and efficiency of Quality Control processes, and enabling the highest quality standards in ADC analysis.
TSKgel® HIC-ADC Column | Porosity | Organic Solvent Tolerance |
| HIC-ADC Butyl | Non-porous | 50% |
| HIC-ADC Phenyl | Non-porous | 50% |
- TSKgel® HIC-ADC Butyl columns contain non-porous particles bonded with butyl ligands. These columns are specifically designed to retain ADC’s, enhancing resolution among variably conjugated ADC isoforms.
- TSKgel® HIC-ADC Phenyl columns are an alternate selectivity option to TSKgel HIC-ADC Butyl suitable for antibody-oligonucleotide conjugates (AOCs) and various hydrophilic targets.