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Cation Exchange Chromatography

TSKgel® Cation Exchange Chromatography Columns

Very efficient chromatography can be achieved with the non-porous TSKgel® STAT columns due to the novel bonding chemistry and the absence of micro-pores. Applications for the TSKgel® CM-STAT and TSKgel® SP-STAT columns include the separation of proteins, protein aggregates,charge variants of monoclonal antibodies, PEGylated proteins, and peptide digests.

The pore structure and bonding chemistry of TSKgel® BioAssist S columns provides high capacity for medium to large molar mass proteins.

TSKgel® CM-5PW and TSKgel® SP-5PW columns are an excellent choice for analyzing biologically active molecules.

TSKgel® SP-NPR (non-porous resin) columns are used for the separation and analysis of proteins and peptides. They are particularly useful for adeno-associated viruses and other large macromolecules.

TSKgel® CM-2SW and TSKgel® SP-2SW (both 5 µm, 12.5 nm) and TSKgel® CM-3SW (10 µm, 25 nm) are available for analyzing smaller molar mass samples such as nucleotides, drug candidates, catecholamines, and small peptides or proteins.

TSKgel® SCX columns are used for the separation and analysis of organic acids, saccharides and alcohols.

TSKgel® OApak-A columns are optimized for the separation and analysis of organic acids by an ion-exclusion mechanism. Applications include: organic acids in fruit juices, wine, beer, coffee, and salt solutions.

Base Material: polymethacrylate
Particle Size (mean): 7 µm and 13 µm  
Pore Size (mean): 130 nm  
Functional Group: sulfopropyl                   Visit the TSKgel® BioAssist S product page.
Counter Ion: Na+          
pH Range: 2.0 - 12.0
Capacity 
(gamme globulin):		 70  
Small Ion Capacity: 0.1 meq/mL
pKa: 2.4

Base Material: spherical silica
Particle Size (mean): 5 µm  
Pore Size (mean): 12.5 nm  
Functional Group: -CH2COO-                             Visit the TSKgel® CM-2SW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):		 110  
Small Ion Capacity: >0.3 meq/mL
pKa: 4.2

Base Material: spherical silica
Particle Size (mean): 10 µm  
Pore Size (mean): 25 nm  
Functional Group: -CH2COO-
                             Visit the TSKgel® CM-3SW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):		 ND  
Small Ion Capacity: >0.3 meq/mL
pKa: 4.2

Base Material: hydroxylated methacrylic
polymer
Particle Size (mean): 10 µm and 13 µm  
Pore Size (mean): 100 nm  
Functional Group: -CH2COO-
                             Visit the TSKgel® CM-5PW product page.
Counter Ion: Na+          
pH Range: 2.0 - 12.0
Capacity 
(mg Hb/mL):		 45  
Small Ion Capacity: >0.1 meq/mL
pKa: 4.2

Base Material: hydrophilic polymer
Particle Size (mean): 7 µm and 10 µm  
Pore Size (mean): non-porous  
Functional Group: carboxymethyl                Visit the TSKgel® CM-STAT product page.
Counter Ion: Na          
pH Range: 3.0 - 10.0
Static Binding Capacity 
(mg Lysozyme/g dry gel):		 ca. 20 (7 µm)
ca. 15 (10 µm)		  
Small Ion Capacity: 100 µeq/g dry gel
pKa: 4.9
Hardware: stainless steel
tubing and fittings, PEEK frits		  

Base Material: hydrophilic polymer
Particle Size (mean): 5 µm  
Pore Size (mean): ND  
Functional Group: proprietary                             Visit the TSKgel® OApak-A product page.
Counter Ion: H+          
pH Range: 2.0 - 7.5
Small Ion Capacity: >1.5 meq/mL

Base Material: PS-DVB
Particle Size (mean): 5 µm  
Pore Size (mean): 6.0 nm  
Functional Group: sulfonic acid                   Visit the TSKgel® SCX (H+) or TSKgel SCX (Na+) product page.
Counter Ion: H+ and Na+          
pH Range: 2 - 12
Small Ion Capacity: >1.5 meq/mL

Base Material: spherical silica
Particle Size (mean): 5 µm  
Pore Size (mean): 12.5 nm  
Functional Group: sulfopropyl                             Visit the TSKgel® SP-2SW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):		 ND  
Small Ion Capacity: >0.3 meq/mL
pKa: 2.2

Base Material: hydroxylated methacrylic
polymer
Particle Size (mean): 10, 13, 20 µm  
Pore Size (mean): 100 nm  
Functional Group: -(CH2)SO3-
                    Visit the TSKgel® SP-5PW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):		 40  
Small Ion Capacity: >0.1 meq/mL
pKa: 2.3

Base Material: hydroxylated methacrylic
 polymer
Particle Size (mean): 2.5 µm  
Pore Size (mean): non-porous  
Functional Group: -(CH2)SO3-
                          Visit the TSKgel® SP-NPR product page.
Counter Ion: Na+
          
pH Range: 2.0 - 12.0
Capacity 
(mg Hb/mL):		 5  
Small Ion Capacity: >0.1 meq/mL  
pKa: 2.3

Base Material: hydrophilic polymer
Particle Size (mean): 7 µm and 10 µm  
Pore Size (mean): non-porous  
Functional Group: sulfopropyl                    Visit the TSKgel® SP-STAT product page.
Counter Ion: Na          
pH Range: 3.0 - 10.0
Static Binding
Capacity (mg
Lysozyme/g dry gel):		 ca. 15 (7 µm)
ca. 10 (10 µm)		  
Small Ion Capacity: 23 µeg/g dry gel  
pKa: 4.0  
Hardware: Stainless Steel
tubing & fittings, 
PEEK frits