Solutions

TSKgel® UP-SW Columns

TSKgel® UP-SW Columns

The TSKgel® UP-SW product line consists of three columns differing in pore size, which allows for the analysis of small to very large molecular weight biopharma products. Available in 4.6 mm ID x 15 and 30 cm lengths, TSKgel® UP-SW columns are compatible with both UHPLC and HPLC systems and require less sample while delivering higher sensitivity compared to 7.8 mm ID TSKgel SEC columns.

The TSKgel® UP-SW columns are:

  • TSKgel® UP-SW2000, 12.5 nm pore size, for analysis of small proteins, peptides and oligonucleotides
  • TSKgel® UP-SW3000, 25 nm pore size, for analysis of mAb monomer and antibody fragments
  • TSKgel® UP-SW Aggregate, 30 nm pore size, for analysis of very large proteins and high order mAb aggregates

As shown in the figure, the calibration curves show differing slopes that create distinct separation ranges for each of the columns within the TSKgel® UP-SW product line. The calibration curve for the TSKgel® UP-SW2000 column is similar to that of the TSKgel® G2000SWXL column curve, implying a better resolution of small proteins, peptides and oligonucleotides. The TSKgel® UP-SW3000 column shows a shallower slope around the molecular weight region of γ-globulin, making this column ideal for the analysis of monoclonal antibodies. The UP-SW Aggregate column demonstrates the shallowest slope of the calibration curve of proteins up to the high molecular weight region.

SEC_UP-SW_calibrations-curves.png
Columns: A. TSKgel® UP-SW2000, 2 µm,
     4.6 mm ID × 30 cm
B. TSKgel® UP-SW3000, 2 µm,
     4.6 mm ID × 30 cm
C. TSKgel® UP-SW Aggregate, 3 µm,
     4.6 mm ID × 30 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7, + 100 mmol/L Na2SO4 + 0.05% NaN3
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Samples: 1. thyroglobulin (MW 640,000 Da)
    (1' thyroglobulin dimer)
2. γ-globulin (MW 155,000 Da)
    (2' γ-globulin dimer)
3. ovalbumin (MW 47,000 Da)
4. riboneclease A (MW 13,000 Da)
5. p-aminobenzoic acid (MW 137 Da)

 

Base Material: silica   
Particle Size (mean): 2 µm  
Pore Size (mean): 12.5 nm  
Functional Group: Diol  Visit the TSKgel® UP-SW2000 product page.
pH Stability: 2.5 - 7.5               
Calibration Range
( proteins):
5,000 - 1.5 × 105 Da  
   

Base Material: silica   
Particle Size (mean): 2 µm  
Pore Size (mean): 25 nm  
Functional Group: Diol  Visit the TSKgel® UP-SW3000 product page.
pH Stability: 2.5 - 7.5               
Calibration Range
(proteins):
10 - 500 kDa  
   

Base Material: silica   
Particle Size (mean): 3 µm  
Pore Size (mean): 30 nm  
Functional Group: Diol  Visit the TSKgel® UP-SW Aggregate product page.
pH Stability: 2.5 - 7.5               
Calibration Range
(globular proteins):
10 - 2,000 kDa